Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody [SZ80-07]
cat.: ET1603-8
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, ICC/IF, IHC-P, FC
Clonality: Monoclonal
Clone number: SZ80-07
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 48 kDa
Isotype: IgG
Immunogen: Recombinant protein within mouse Cytokeratin 18 aa 200-423.
Positive control: A431 cell lysate, human lung tissue lysate, Hela, A431, HepG2, human colon carcinoma tissue, human breast carcinoma tissue, human colon tissue,human liver tissue, human stomach tissue, human stomach carcinoma tissue, rat skin tissue, MCF-7.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC

1:500-1:2,000
1:100-1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P05783 Human | P05784 Mouse | Q5BJY9 Rat
Alternative names: Cell proliferation inducing gene 46 protein Cell proliferation inducing protein 46 Cell proliferation-inducing gene 46 protein CK 18 CK-18 CK18 CYK 18 CYK18 Cytokeratin 18 Cytokeratin endo B Cytokeratin-18 K 18 K18 K1C18_HUMAN KA18 Keratin 18 Keratin 18, type I Keratin D keratin, type I cytoskeletal 18 Keratin-18 Krt18
Images
ET1603-8_1.jpg Fig1: Western blot analysis of Cytokeratin 18 on different lysates with
Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/500 dilution.

Lane 1: A431 cell lysate,
Lane 2: Human lung tissue lysate

Lysates/proteins at 10 µg/Lane.

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1603-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1603-8_2.jpg Fig2: Immunocytochemistry analysis of Hela cells labeling
Cytokeratin 18 with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/100
dilution.

Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-8, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-8_3.jpg Fig3: Immunocytochemistry analysis of A431 cells labeling
Cytokeratin 18 with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/100
dilution.

Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-8, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-8_4.jpg Fig4: Immunocytochemistry analysis of HepG2 cells labeling
Cytokeratin 18 with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/100
dilution.

Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1603-8, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1603-8_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded human colon carcinoma with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Cytokeratin 18 antibody (ET1603-8) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1603-8, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1603-8_13.jpg Fig13: Flow cytometric analysis of Cytokeratin 18 was done on MCF-7 cells.

The cells were fixed, permeabilized and stained with the primary antibody (ET1603-8, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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