FOXO3A Recombinant Rabbit Monoclonal Antibody [SP0054]
cat.: ET1604-11
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue
Clonality: Monoclonal
Clone number: SP0054
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 71 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human FOXO3A aa 21-69/673.
Positive control: NIH/3T3 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, Hela, MCF-7,SW480.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue

1:1,000-1:5,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: O43524 Human | Q9WVH4 Mouse
Entrez Gene: 294515 Rat
Alternative names: AF6q21 AF6q21 protein DKFZp781A0677 FKHR2 FKHRL 1 FKHRL1 FKHRL1P2 Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 Forkhead box O3 Forkhead box O3A Forkhead box protein O3 Forkhead box protein O3A Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 Forkhead homolog (rhabdomyosarcoma) like 1 Forkhead in rhabdomyosarcoma like 1 Forkhead in rhabdomyosarcoma-like 1 FOX O3A FOXO2 foxo3 FOXO3_HUMAN FOXO3A MGC12739 MGC31925
Images
ET1604-11_1.jpg Fig1: Western blot analysis of FOXO3A on different lysates with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/1,000 dilution.

Lane 1: NIH/3T3 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: MCF-7 cell lysate

Lysates/proteins at 10 µg/Lane.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-11) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1604-11_2.jpg Fig2: Immunocytochemistry analysis of Hela cells labeling FOXO3A with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃ , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1604-11_3.jpg Fig3: Immunocytochemistry analysis of MCF-7 cells labeling FOXO3A with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃ , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
ET1604-11_4.jpg Fig4: Immunocytochemistry analysis of SW480 cells labeling FOXO3A with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃ , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.