FOXO3A Recombinant Rabbit Monoclonal Antibody [SP0054]
cat.: ET1604-11
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SP0054 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human FOXO3A aa 21-69/673. |
| Positive control: | NIH/3T3 cell lysate, MCF-7 cell lysate, PC-12 cell lysate, Hela, MCF-7,SW480. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000-1:5,000 1:100-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: O43524 Human | Q9WVH4 Mouse Entrez Gene: 294515 Rat |
| Alternative names: | AF6q21 AF6q21 protein DKFZp781A0677 FKHR2 FKHRL 1 FKHRL1 FKHRL1P2 Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 Forkhead box O3 Forkhead box O3A Forkhead box protein O3 Forkhead box protein O3A Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 Forkhead homolog (rhabdomyosarcoma) like 1 Forkhead in rhabdomyosarcoma like 1 Forkhead in rhabdomyosarcoma-like 1 FOX O3A FOXO2 foxo3 FOXO3_HUMAN FOXO3A MGC12739 MGC31925 |
Images
|
Fig1:
Western blot analysis of FOXO3A on different lysates with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 71 kDa Observed band size: 82 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-11) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of MCF7 cells labeling FOXO3A with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FOXO3A antibody (ET1604-11) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.