GFP Recombinant Rabbit Monoclonal Antibody [SP00-68]
cat.: ET1604-25
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Species independent
Applications: WB, IHC-P, IF-Cell, IF-Tissue, IP
Clonality: Monoclonal
Clone number: SP00-68
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 27 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.
Positive control: K562 cell lysates transfected with GFP.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  IP

1:10,000-1:50,000
1:500-1:1,000
1:500-1:1,000
1:500-1:1,000
2-5 µg/ml.
Uniprot #: SwissProt: P42212 AequoreaVictoria
Alternative names: GFP Green fluorescent protein yfp
Images
ET1604-25_1.jpg Fig1: Western blot analysis of GFP on K562 cell lysates
transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-25, 1/50,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1604-25_2.jpg Fig2: GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with ET1604-25 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.

Lane 1: GFP Tag fusion protein lysate (input).
Lane 2: Rabbit IgG instead of ET1604-25 in GFP Tag fusion protein lysate.
Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate.
Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate.
Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate.
Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate.
Lane 7: R1312-2 IP in GFP Tag fusion protein lysate.

Blocking/Dilution buffer: 5% NFDM/TBST
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.