Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Species independent |
Applications: | WB, IHC-P, IF-Cell, IF-Tissue, IP |
Clonality: | Monoclonal |
Clone number: | SP00-68 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 27 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238. |
Positive control: | K562 cell lysates transfected with GFP. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:10,000-1:50,000 1:500-1:1,000 1:500-1:1,000 1:500-1:1,000 2-5 µg/ml. |
Uniprot #: | SwissProt: P42212 AequoreaVictoria |
Alternative names: | GFP Green fluorescent protein yfp |
Fig1:
Western blot analysis of GFP on K562 cell lysates transfected with GFP. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-25, 1/50,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2:
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with ET1604-25 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: GFP Tag fusion protein lysate (input). Lane 2: Rabbit IgG instead of ET1604-25 in GFP Tag fusion protein lysate. Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate. Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate. Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate. Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate. Lane 7: R1312-2 IP in GFP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |