Anti-Olig2 antibody [SP07-02]
cat.: ET1604-29
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SP07-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 32 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Olig2 aa 238-287 / 323.
Positive control: HL-60 cell lysate, THP-1 cell lysate, rat brain tissue, mouse brain tissue.
Subcellular location: Nucleus, Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P

1:500- 1:1,000
1:50-1:200
Uniprot #: SwissProt: Q13516 Human | Q9EQW6 Mouse
Unigene: 22121 Rat
Alternative names: Basic domain helix loop helix protein class B 1 Basic helix loop helix protein class B 1 BHLHB bHLHB1 bHLHe19 Class B basic helix loop helix protein 1 Class B basic helix-loop-helix protein 1 class E basic helix loop helix protein 19 Class E basic helix-loop-helix protein 19 Human protein kinase C binding protein RACK17 Olig2 OLIG2_HUMAN Oligo2 Oligodendrocyte lineage transcription factor 2 Oligodendrocyte specific bHLH transcription factor 2 Oligodendrocyte transcription factor 2 OTTHUMP00000067569 OTTHUMP00000067570 PRKCBP2 Protein kinase C binding protein 2 Protein kinase C binding protein RACK17 Protein kinase C-binding protein 2 Protein kinase C-binding protein RACK17 RACK17
Images
ET1604-29_1.jpg Fig1: Western blot analysis of Olig2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1604-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: THP-1 cell lysate
ET1604-29_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Olig2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1604-29_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Olig2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.