ERK1 Recombinant Rabbit Monoclonal Antibody [SP00-74]
cat.: ET1604-32
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SP00-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human ERK1. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, Ramos cell lysate, MCF7 cell lysate, Jurkat, human breast cancer tissue, human stomach tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P FC |
1:1,000-1:5,000 1:100 1:100-1:500 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P27361 Human |
| Alternative names: | ERK 1 ERK ERK-1 ERK1 ERT 2 ERT2 Extracellular Signal Regulated Kinase 1 Extracellular signal related kinase 1 Extracellular signal-regulated kinase 1 HGNC6877 HS44KDAP HUMKER1A Insulin Stimulated MAP2 Kinase Insulin-stimulated MAP2 kinase MAP kinase 1 MAP kinase 3 MAP Kinase MAP kinase isoform p44 MAPK 1 MAPK 3 MAPK MAPK1 Mapk3 MGC20180 Microtubule Associated Protein 2 Kinase Microtubule-associated protein 2 kinase Mitogen Activated Protein Kinase 3 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 3 MK03_HUMAN OTTHUMP00000174538 OTTHUMP00000174541 p44 ERK1 p44 MAPK p44-ERK1 p44-MAPK P44ERK1 P44MAPK PRKM 3 PRKM3 Protein Kinase Mitogen Activated 3 |
Images
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Fig1:
Western blot analysis of ERK1 on different lysates with Rabbit anti-ERK1 antibody (ET1604-32) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: Ramos cell lysate Lane 5: MCF7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-32) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling ERK1 with Rabbit anti-ERK1 antibody (ET1604-32) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK1 antibody (ET1604-32) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-ERK1 antibody (ET1604-32) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-32) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-ERK1 antibody (ET1604-32) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-32) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Flow cytometric analysis of Jurkat cells labeling ERK1. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1604-32, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.