RNF20 Recombinant Rabbit Monoclonal Antibody [SP00-47]
cat.: ET1604-4
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SP00-47 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 114 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RNF20 aa 480-585/975. |
| Positive control: | HeLa cell lysate, MCF7 cell lysate, C2C12 cell lysate, A549. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q5VTR2 Human | Q5DTM8 Mouse |
| Alternative names: | BRE1 A BRE1 BRE1 E3 ubiquitin ligase homolog BRE1-A BRE1A BRE1A_HUMAN E3 ubiquitin-protein ligase BRE1A hBRE1 Homolog of S. cerevisiae BRE1 RING finger protein 20 Ring finger protein 20 E3 ubiquitin protein ligase Rnf20 |
Images
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Fig1:
Western blot analysis of RNF20 on different lysates with Rabbit anti-RNF20 antibody (ET1604-4) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 114 kDa Observed band size: 114 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Flow cytometric analysis of RNF20 was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1604-4, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). |
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Fig3:
Immunocytochemistry analysis of A549 cells labeling RNF20 with Rabbit anti-RNF20 antibody (ET1604-4) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RNF20 antibody (ET1604-4) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.