Heme Oxygenase 1(HO-1) Recombinant Rabbit Monoclonal Antibody [SP08-07]
cat.: ET1604-45
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP, FC, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SP08-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 33 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HO-1 aa 227-276 / 288. |
| Positive control: | Human spleen tissue, human liver tissue, Jurkat. |
| Subcellular location: | Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IHC-P FC IP IF-Tissue |
1:1,000 1:50-1:1,000 1:500-1:1,000 Use at an assay dependent concentration. 1:50-1:200 |
| Uniprot #: | SwissProt: P09601 Human |
| Alternative names: | 32 kD bK286B10 D8Wsu38e heat shock protein 32 kD heat shock protein 32kD Heat shock protein Heme oxygenase (decycling) 1 Heme oxygenase 1 Hemox HMOX 1 Hmox Hmox1 HMOX1_HUMAN HO 1 HO HO-1 HO1 Hsp32 |
Images
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Fig1:
Flow cytometric analysis of Jurkat cells labeling Heme Oxygenase 1(HO-1). Cells were fixed and permeabilized. Then stained with the primary antibody (ET1604-45, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (ET1604-45) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Heme Oxygenase 1(HO-1) antibody (ET1604-45) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1604-45) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.