Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SY88-19]
cat.: ET1605-17
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SY88-19 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 62 kDa |
| Isotype: | IgG |
| Immunogen: | recombinant protein |
| Positive control: | A431 cell lysates, human tonsil tissue, human lung cancer tissue, human breast cancer tissue. |
| Subcellular location: | Cytoplasm,nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P13647 Human | Q922U2 Mouse |
| Alternative names: | 58 kDa cytokeratin CK-5 CK5 Cytokeratin-5 Cytokeratin5 DDD DDD1 EBS2 epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types K2C5_HUMAN K5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) Keratin 5 Keratin keratin complex 2, basic, gene 5 keratin, type II cytoskeletal 5 Keratin-5 Keratin5 KRT 5 Krt5 KRT5A type II cytoskeletal 5 Type-II keratin Kb5 |
Images
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Fig1:
Western blot analysis of Cytokeratin 5 on A431 cell lysates with Rabbit anti-Cytokeratin 5 antibody (ET1605-17) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 62 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-17) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human brest cancer tissue using anti-Cytokeratin 5 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.