TACC3 Recombinant Rabbit Monoclonal Antibody [SY10-06]
cat.: ET1605-18
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P
Clonality: Monoclonal
Clone number: SY10-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 90 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human TACC3 aa 40-89 / 838.
Positive control: HT29 cell lysates, Hela cell lysates,Hela, HepG2, human tonsil tissue, human spleen tissue, mouse testis tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P

1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q9Y6A5 Human | Q9JJ11 Mouse
Unigene: 58838 Rat
Alternative names: ERIC 1 ERIC-1 ERIC1 MGC117382 MGC133242 OTTHUMP00000113796 TACC3 TACC3_HUMAN Transforming acidic coiled coil containing protein 3 Transforming acidic coiled-coil-containing protein 3
Images
ET1605-18_1.jpg Fig1: Western blot analysis of TACC3 on different lysates with Rabbit anti-TACC3 antibody (ET1605-18) at 1/500 dilution.

Lane 1: HT29 cell lysates
Lane 2: Hela cell lysates

Lysates/proteins at 10 µg/Lane.

Predicted band size: 90 kDa
Observed band size: 140 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-18) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ET1605-18_2.jpg Fig2: ICC staining TACC3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1605-18_3.jpg Fig3: ICC staining TACC3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1605-18_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
ET1605-18_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TACC3 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.