ET1605-20

PDGF Receptor beta Recombinant Rabbit Monoclonal Antibody [SY10-08]

cat.: ET1605-20

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IP, FC, IF-Tissue, IF-Cell, mIHC
Clonality:	Monoclonal
Clone number:	SY10-08

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size:123 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human PDGF Receptor beta aa 961-1,106 / 1,106.
Positive control:	NIH/3T3 cell lysates, human lung tissue, human spleen tissue, mouse lung tissue, mouse brain tissue, human tonsils tissue, mouse spleen tissue, mouse kidney tissue.
Subcellular location:	Cell membrane, Cytoplasmic vesicle, Lysosome lumen.
Recommended Dilutions: WB IHC-P FC IF-Tissue IF-Cell mIHC	1:500-1:2,000 1:50-1:200 1:1,000 1:200 : 1:100 1:1,500-1:2,000
Uniprot #:	SwissProt: P05622 Mouse \| P09619 Human \| Q05030 Rat
Alternative names:	Beta platelet derived growth factor receptor Beta-type platelet-derived growth factor receptor CD 140B CD140 antigen-like family member B CD140b CD140b antigen IBGC4 IMF1 JTK12 OTTHUMP00000160528 PDGF R beta PDGF-R-beta PDGFR 1 PDGFR PDGFR beta PDGFR1 PDGFRB PGFRB_HUMAN Platelet derived growth factor receptor 1 Platelet derived growth factor receptor beta Platelet derived growth factor receptor beta polypeptide

Images

Fig1: Western blot analysis of PDGF Receptor beta on different lysates with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/1,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: C6 cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain tissue lysate

Lysates/proteins at 20 (cell) or 40 (tissue) µg/Lane.

Predicted band size: 150/200 kDa
Observed band size: 190 kDa

Exposure time: 6 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-20) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Immunocytochemistry analysis of SH-SY5Y cells labeling PDGF Receptor beta with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

	Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1605-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1605-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1605-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1605-20) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of SH-SY5Y cells labeling PDGF Receptor beta. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1605-20, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
	Fig8: mIHC analysis of human tonsils tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/1,500 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
	Fig9: mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/2,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Fig10: mIHC analysis of mouse kidney tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-PDGF Receptor beta antibody (ET1605-20) at 1/2,000 dilution. The immunostaining was performed with the IRISKit® HyperView mTSA Kit (MH900206). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.