Anti-ALDH1A1 antibody [SY11-02]
cat.: ET1605-24
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SY11-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 55 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ALDH1A1 aa 300-360.
Positive control: Mouse lung tissue lysate, human liver tissue lysate, human kidney tissue lysate, human liver tissue, human kidney tissue, mouse brain tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  IP

1:1,000-1:2,000
1:50-1:200
Use at an assay dependent concentration.
Uniprot #: SwissProt: P00352 Human | P24549 Mouse | P51647 Rat
Alternative names: Acetaldehyde dehydrogenase 1 AHD2 AL1A1_HUMAN ALDC Aldehyde dehydrogenase 1 family member A1 Aldehyde dehydrogenase 1 soluble Aldehyde dehydrogenase 1A1 Aldehyde dehydrogenase Aldehyde dehydrogenase cytosolic Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase liver cytosolic ALDH 1 ALDH 1A1 ALDH class 1 ALDH, liver cytosolic ALDH-E1 ALDH1 A1 ALDH1 ALDH11 ALDH1A1 ALHDII cytosolic epididymis luminal protein 12 epididymis luminal protein 9 epididymis secretory sperm binding protein Li 53e HEL-S-53e MGC2318 PUMB1 RALDH 1 RalDH1 Retinal dehydrogenase 1
Images
ET1605-24_1.jpg Fig1: Western blot analysis of ALDH1A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse lung tissue lysate
Lane 2: human liver tissue lysate
Lane 3: human kidney tissue lysate
ET1605-24_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ALDH1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-24_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ALDH1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-24, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-24_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ALDH1A1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.