SHIP Recombinant Rabbit Monoclonal Antibody [SY11-08]
cat.: ET1605-30
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SY11-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 133 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SHIP aa 1,064-1,189 / 1,189. |
| Positive control: | HL-60 cell lysate, Daudi cell lysate, human tonsil tissue, human spleen tissue, Raji. |
| Subcellular location: | Cytoskeleton, Cell membrane, Cytoplasm, Membrane raft, Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q92835 Human |
| Alternative names: | Inositol polyphosphate 5 phosphatase of 145kDa 4 5-trisphosphate 5-phosphatase 1 hp51CN Inositol polyphosphate 5 phosphatase 145kDa Inositol polyphosphate 5 phosphatase Inositol polyphosphate-5-phosphatase of 145 kDa INPP5D MGC104855 MGC142140 MGC142142 p150Ship Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 Phosphatidylinositol-3 SH2 containing inositol phosphatase isoform b SH2 domain containing inositol 5' phosphatase 1 SH2 domain containing inositol phosphatase 1 SH2 domain-containing inositol phosphatase 1 SH2 domain-containing inositol-5'-phosphatase 1 SHIP-1 SHIP1 SHIP1_HUMAN Signaling inositol polyphosphate 5 phosphatase SIP 145 SIP-145 SIP145 |
Images
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Fig1:
Western blot analysis of SHIP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-30, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SHIP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-SHIP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of SHIP was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1605-30, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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