CD20 Recombinant Rabbit Monoclonal Antibody [SY12-01]
cat.: ET1605-33
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Monkey
Applications: WB, IHC-P, IP
Clonality: Monoclonal
Clone number: SY12-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 33 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD20 aa 248-297 / 297.
Positive control: Raji cell lysates, human tonsil tissue, human spleen tissue, B-cell lymphoma tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P

1:1,000-1:5,000
1:100-1:500
Uniprot #: SwissProt: P11836 Human
Alternative names: APY ATOPY B lymphocyte antigen CD20 B Lymphocyte Cell Surface Antigen B1 B-lymphocyte antigen CD20 B-lymphocyte cell-surface antigen B1 B-lymphocyte surface antigen B1 B1 Bp 35 Bp35 CD 20 CD20 CD20 antigen CD20 receptor CD20_HUMAN CVID5 Fc epsilon receptor I beta chain Fc Fragment of IgE high affinity I receptor for beta polypeptide FCER1B High affinity immunoglobulin epsilon receptor subunit beta IgE Fc receptor subunit beta IGEL IGER IGHER LEU 16 LEU16 leukocyte surface antigen Leu 16 Leukocyte surface antigen Leu-16 Ly44 Membrane spanning 4 domains A1 Membrane spanning 4 domains subfamily A member 2 Membrane-spanning 4-domains subfamily A member 1 MS4A1 MS4A2 S7
Images
ET1605-33_1.jpg Fig1: Western blot analysis of CD20 on Raji cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1605-33_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-33, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-33_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-33, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-33_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded B-cell lymphoma tissue using anti-CD20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-33, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.