Anti-HDAC1 antibody [SY12-04]
cat.: ET1605-35
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SY12-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 65 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human HDAC1 aa 420-460.
Positive control: MCF-7 cell lysate, Hela cell lysate, Jurkat cell lysate, K562 cell lysate, human colon tissue, mouse colon tissue, mouse pancreas tissue, human pancreas tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: Q13547 Human | O09106 Mouse | Q4QQW4 Rat
Alternative names: DKFZp686H12203 GON 10 HD1 HDAC 1 HDAC1 HDAC1_HUMAN Histone deacetylase 1 Reduced potassium dependency yeast homolog like 1 RPD3 RPD3L1
Images
ET1605-35_1.jpg Fig1: Western blot analysis of HDAC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1605-35, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 2: MCF-7 cell lysate
Lane 2: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 2: K562 cell lysate
ET1605-35_2.jpg Fig2: All lanes: Western blot analysis of HDAC1 with anti-HDAC1 antibody [SY12-04] (ET1605-35) at 1:1,000 dilution.

Lane 1: Wild-type HepG2 whole cell lysate (10µg).
Lane 2: HDAC1 knockout HepG2 whole cell lysate (10µg).

Secondary antibody: Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:10,000 dilution.

Exposure time: 1 minute for all lanes.

Loading control: Rabbit anti-HSP90 at 1/10,000 dilution.

Blocking agent: 5% NFDM/TBST.
ET1605-35_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-HDAC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-35, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-35_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HDAC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-35, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-35_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-HDAC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-35, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-35_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-HDAC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-35, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.