CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02]
cat.: ET1605-4
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, FC, CUT&Tag-seq
Clonality: Monoclonal
Clone number: SY09-02
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 34 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CDX2 aa 20-60.
Positive control: Caco-2 cell lysate, LoVo cell lysate, AGS cell lysate, LOVO, AGS, human appendix tissue, Human stomach cancer tissue, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue, Hela.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
1:1,000-1:2,000
1:50-1:200
1:200-1:2,000
1:500-20,000
1:50-1:100
Uniprot #: SwissProt: Q99626 Human | P43241 Mouse
Entrez Gene: 66019 Rat
Alternative names: Caudal type homeo box 2 Caudal type homeo box transcription factor 2 Caudal type homeobox 2 Caudal type homeobox protein 2 Caudal type homeobox transcription factor 2 Caudal-type homeobox protein 2 CDX 2 CDX 3 CDX-3 Cdx2 CDX2_HUMAN CDX3 Homeobox protein CDX 2 Homeobox protein CDX-2
Images
ET1605-4_1.jpg Fig1: Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (ET1605-4) at 1/1,000 dilution.

Lane 1: Caco-2 cell lysate
Lane 2: LoVo cell lysate
Lane 3: AGS cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 25 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1605-4_2.jpg Fig2: Western blot analysis of CDX2 on different lysates with Rabbit anti-CDX2 antibody (ET1605-4) at 1/1,000 dilution.

Lane 1: MCF7-si NT cell lysate
Lane 2: MCF7-si CDX2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1605-4_3.jpg Fig3: ICC staining of CDX2 in LOVO cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1605-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1605-4_4.jpg Fig4: ICC staining of CDX2 in AGS cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1605-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1605-4_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-CDX2 antibody (ET1605-4) at 1/20,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded human thymus tissue (negative) with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_12.jpg Fig12: Immunohistochemical analysis of paraffin-embedded rat thymus tissue (negative) with Rabbit anti-CDX2 antibody (ET1605-4) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1605-4) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1605-4_13.jpg Fig13: Flow cytometric analysis of CDX2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1605-4, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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