V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01]
cat.: ET1605-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, IP |
| Clonality: | Monoclonal |
| Clone number: | SY30-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is CGKPIPNPLLGLDST. |
| Positive control: | V5 tagged recombinant protein. |
| Recommended Dilutions:
WB IF-Cell IP |
1:1,000-1:5,000 1:2,000 1-2μg/sample |
| Alternative names: | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag Protein Rev Regulator of expression of viral proteins rev V5 epitope tag |
Images
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Fig1:
Western blot analysis of V5 tag on different lysates with Rabbit anti-V5 tag antibody (ET1605-41) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate Lane 3: 293T transfected with V5-tagged Nanos homolog 3 (C-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-41) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling V5 tag with Rabbit anti-V5 tag antibody (ET1605-41) at 1/2,000 dilution. HeLa cells, transfected with V5-tagged empty control, Histone H3 (N-terminal) or Nanos homolog 3 (C-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (ET1605-41) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. V5 tag (M1008-2, red) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
V5 tag was immunoprecipitated from 0.2 mg 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate with ET1605-41 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1605-41 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate (input) Lane 2: ET1605-41 IP in 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate Lane 3: Rabbit IgG instead of ET1605-41 in 293T transfected with V5-tagged Histone H3.1 (N-terminal) cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.