V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01]
cat.: ET1605-41
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SY30-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is CGKPIPNPLLGLDST. |
| Positive control: | V5 tagged recombinant protein. |
| Recommended Dilutions:
WB |
1:1,000-1:5,000 |
| Alternative names: | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag Protein Rev Regulator of expression of viral proteins rev V5 epitope tag |
Images
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Fig1:
Western blot analysis of V5 tag on V5 tagged recombinant protein with Rabbit anti-V5 tag antibody (ET1605-41) at 1/2,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-41) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.