ARG1 Recombinant Rabbit Monoclonal Antibody [SY09-06]
cat.: ET1605-8
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP
Clonality: Monoclonal
Clone number: SY09-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ARG1 aa 20-60.
Positive control: Mouse liver tissue lysate, human liver tissue lysate.
Subcellular location: Cytoplasm,Cytoplasmic granule.
Recommended Dilutions:
  WB
  IP

1:2,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: P05089 Human | Q61176 Mouse | P07824 Rat
Alternative names: arg1 ARGI1_HUMAN Arginase-1 Liver-type arginase Type I arginase
Images
ET1605-8_1.jpg Fig1: Western blot analysis of ARG1 on different lysates with Rabbit anti-ARG1 antibody (ET1605-8) at 1/2,000 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Human liver tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 36 kDa

Exposure time: 30 seconds;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.