Liver Arginase Recombinant Rabbit Monoclonal Antibody [SY09-06]
cat.: ET1605-8
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SY09-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ARG1 aa 20-60. |
| Positive control: | Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate, RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate. |
| Subcellular location: | Cytoplasm,Cytoplasmic granule. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P05089 Human | Q61176 Mouse | P07824 Rat |
| Alternative names: | arg1 ARGI1_HUMAN Arginase-1 Liver-type arginase Type I arginase |
Images
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Fig1:
Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 37 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 37 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.