Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | SY09-06 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 35 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human ARG1 aa 20-60. |
Positive control: | Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate, RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate. |
Subcellular location: | Cytoplasm,Cytoplasmic granule. |
Recommended Dilutions:
WB |
1:1,000-1:2,000 |
Uniprot #: | SwissProt: P05089 Human | Q61176 Mouse | P07824 Rat |
Alternative names: | arg1 ARGI1_HUMAN Arginase-1 Liver-type arginase Type I arginase |
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Fig1:
Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/2,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 37 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 35 kDa Observed band size: 37 kDa Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |