Liver Arginase Recombinant Rabbit Monoclonal Antibody [SY09-06]
cat.: ET1605-8
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: SY09-06
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 35 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ARG1 aa 20-60.
Positive control: Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate, RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate.
Subcellular location: Cytoplasm,Cytoplasmic granule.
Recommended Dilutions:
  WB

1:1,000-1:2,000
Uniprot #: SwissProt: P05089 Human | Q61176 Mouse | P07824 Rat
Alternative names: arg1 ARGI1_HUMAN Arginase-1 Liver-type arginase Type I arginase
Images
ET1605-8_1.jpg Fig1: Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/2,000 dilution.

Lane 1: Human liver tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Rat liver tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 37 kDa

Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1605-8_2.jpg Fig2: Western blot analysis of Liver Arginase on different lysates with Rabbit anti-Liver Arginase antibody (ET1605-8) at 1/1,000 dilution.

Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 185ng/mL TPA for 54 hours add 20ng/mL hIFN-γ and 100ng/mL LPS for 48 hours cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: RAW264.7 treated with 20ng/mL mIL-4 and 10ng/mL mIL-13 for 24 hours cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 37 kDa

Exposure time: Lane 1-2: 59 seconds; Lane 3-4: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-8) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.