Dnmt3b Recombinant Rabbit Monoclonal Antibody [SY09-07]
cat.: ET1605-9
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, ChIP |
| Clonality: | Monoclonal |
| Clone number: | SY09-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Dnmt3b aa 61-110 / 853. |
| Positive control: | NCCIT cell lysates, NCCIT, C6. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell ChIP |
1:2,000 1:50-1:250 Use 2 μg for 25 μg of chromatin. |
| Uniprot #: | SwissProt: Q9UBC3 Human Unigene: 117353 Rat |
| Alternative names: | Cytosine 5methyltransferase 3B DNA DNA (cytosine 5) methyltransferase 3 beta DNA (cytosine 5)-methyltransferase 3B DNA (cytosine-5)-methyltransferase 3B DNA methyltransferase HsaIIIB DNA MTase HsaIIIB DNM3B_HUMAN Dnmt3b EC 2.1.1.37 ICF ICF1 M.HsaIIIB MGC124407 RP23-89H14.3 |
Images
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Fig1:
Western blot analysis of Dnmt3b on NCCIT cell lysates with Rabbit anti-Dnmt3b antibody (ET1605-9) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 96 kDa Observed band size: 81 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-9) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NCCIT cells labeling Dnmt3b with Rabbit anti-Dnmt3b antibody (ET1605-9) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt3b antibody (ET1605-9) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of C6 cells labeling Dnmt3b with Rabbit anti-Dnmt3b antibody (ET1605-9) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dnmt3b antibody (ET1605-9) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4: Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells with Dnmt3b (ET1605-9) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
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Fig5: Chromatin immunoprecipitations were performed with cross-linked chromatin from NCCIT cells with Dnmt3b (ET1605-9) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.