Dnmt3b Recombinant Rabbit Monoclonal Antibody [SY09-07]
cat.: ET1605-9
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | SY09-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 96 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Dnmt3b aa 61-110 / 853. |
| Positive control: | NCCIT cell lysates. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q9UBC3 Human | O88509 Mouse Unigene: 117353 Rat |
| Alternative names: | Cytosine 5methyltransferase 3B DNA DNA (cytosine 5) methyltransferase 3 beta DNA (cytosine 5)-methyltransferase 3B DNA (cytosine-5)-methyltransferase 3B DNA methyltransferase HsaIIIB DNA MTase HsaIIIB DNM3B_HUMAN Dnmt3b EC 2.1.1.37 ICF ICF1 M.HsaIIIB MGC124407 RP23-89H14.3 |
Images
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Fig1:
Western blot analysis of Dnmt3b on NCCIT cell lysates with Rabbit anti-Dnmt3b antibody (ET1605-9) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 96 kDa Observed band size: 90-100 kDa Exposure time: 1 minute 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-9) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.