Phospho-p53 (S392) Recombinant Rabbit Monoclonal Antibody [SI17-04]
cat.: ET1606-24
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SI17-04 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser392 of Human p53 aa 344-393 / 393. |
| Positive control: | RAW264.7 cell lysate, PC-12 cell lysate, human stomach carcinoma tissue, mouse prostate tissue, A549 cells. |
| Subcellular location: | Cytoplasm, Nucleus, Endoplasmic reticulum, Mitochondrion matrix. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000-1:5,000 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P04637 Human | P02340 Mouse | P10361 Rat |
| Alternative names: | Antigen NY-CO-13 BCC7 Cellular tumor antigen p53 FLJ92943 LFS1 Mutant tumor protein 53 p53 p53 tumor suppressor P53_HUMAN Phosphoprotein p53 Tp53 Transformation related protein 53 TRP53 Tumor protein 53 Tumor protein p53 Tumor suppressor p53 |
Images
|
Fig1:
Western blot analysis of Phospho-p53(S392) on 293 cell lysates. Lane 1: 293 cells, whole cell lysate, 10ug/lane Lane 2: 293 cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-p53(S392) antibody (ET1606-24) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 53 kDa Observed band size: 53 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 10 seconds |
|
Fig2:
Western blot analysis of Phospho-p53(S392) on A549 cell lysates. Lane 1: A549 cells, whole cell lysate, 10ug/lane Lane 2/3: A549 cells treated with 250nM Doxorubicin overnight, whole cell lysates, 10ug/lane Lane 4: A549 cells treated with 250nM Doxorubicin overnight, then treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Phospho-p53(S392) antibody (ET1606-24) at 1:500 dilution. Anti-GAPDH antibody (ET1601-4) at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) (HA1001) at 1/200,000 dilution. Predicted band size: 53 kDa Observed band size: 53 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 5 minutes |
|
Fig3:
Western blot analysis of Phospho-p53 (S392) on different lysates with Rabbit anti-Phospho-p53 (S392) antibody (ET1606-24) at 1/1,000 dilution. Lane 1: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate Lane 2: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate, the membrane treated with λpp for 1 hour Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Exposure time: 142s ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1606-24, 1/1,000 in NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 43.7 kDa Observed band size: 53 kDa |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Phospho-p53 (S392) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Phospho-p53 (S392) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue untreaed and treated with λ-PPase with Rabbit anti-Phospho-p53 (S392) antibody (ET1606-24) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-24) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.