Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IF-Cell, IF-Tissue, IP, IHC-P |
Clonality: | Monoclonal |
Clone number: | SJ0186 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 124 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human PIK3CA aa 1,001-1,050 / 1,068. |
Positive control: | HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, HepG2 cells, Hela cells, MCF-7 cells, human brain tissue, human liver tissue, mouse liver tissue. |
Subcellular location: | Cytosol, plasma membrane, cytoplasm, lamellipodium, membrane, phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase complex, class IA. |
Recommended Dilutions:
WB IF-Cell IF-Tissue IP IHC-P |
1:1,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. 1:50-1:200 |
Uniprot #: | SwissProt: P42336 Human | P42337 Mouse |
Alternative names: | 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha 5-bisphosphate 3-kinase catalytic subunit alpha isoform caPI3K CLOVE CWS5 MCAP MCM MCMTC MGC142161 MGC142163 p110 alpha p110alpha Phosphatidylinositol 3 kinase catalytic alpha polypeptide Phosphatidylinositol 3 kinase catalytic 110 KD alpha Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha Phosphatidylinositol-4 Phosphoinositide 3 kinase catalytic alpha polypeptide PI3 kinase p110 subunit alpha PI3-kinase subunit alpha PI3K PI3K-alpha PI3KC A PIK3C A Pik3ca PK3CA PK3CA_HUMAN PtdIns 3 kinase p110 PtdIns-3-kinase subunit alpha PtdIns-3-kinase subunit p110-alpha Serine/threonine protein kinase PIK3CA |
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Fig1:
Western blot analysis of PI3 Kinase p110α on different lysates with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: Jurkat cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: mouse brain tissue lysate(20 µg/Lane) Lane 6: mouse liver tissue lysate(20 µg/Lane) Lysates/proteins at 10 µg/Lane. Predicted band size: 124 kDa Observed band size: 110 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of PI3 Kinase p110α in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of PI3 Kinase p110α in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of PI3 Kinase p110α in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-36) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |