PI3 Kinase p110α Recombinant Rabbit Monoclonal Antibody [SJ0186]
cat.: ET1606-36
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IP, IHC-P
Clonality: Monoclonal
Clone number: SJ0186
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 124 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PIK3CA aa 1,001-1,050 / 1,068.
Positive control: HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, HepG2 cells, Hela cells, MCF-7 cells, human brain tissue, human liver tissue, mouse liver tissue.
Subcellular location: Cytosol, plasma membrane, cytoplasm, lamellipodium, membrane, phosphatidylinositol 3-kinase complex, phosphatidylinositol 3-kinase complex, class IA.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IP
  IHC-P
1:1,000
1:50-1:200
1:50-1:200
1-2μg/sample
1:50-1:200
Uniprot #: SwissProt: P42336 Human | P42337 Mouse
Alternative names: 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha 5-bisphosphate 3-kinase catalytic subunit alpha isoform caPI3K CLOVE CWS5 MCAP MCM MCMTC MGC142161 MGC142163 p110 alpha p110alpha Phosphatidylinositol 3 kinase catalytic alpha polypeptide Phosphatidylinositol 3 kinase catalytic 110 KD alpha Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha Phosphatidylinositol-4 Phosphoinositide 3 kinase catalytic alpha polypeptide PI3 kinase p110 subunit alpha PI3-kinase subunit alpha PI3K PI3K-alpha PI3KC A PIK3C A Pik3ca PK3CA PK3CA_HUMAN PtdIns 3 kinase p110 PtdIns-3-kinase subunit alpha PtdIns-3-kinase subunit p110-alpha Serine/threonine protein kinase PIK3CA
Images
ET1606-36_1.jpg Fig1: Western blot analysis of PI3 Kinase p110α on different lysates with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: Jurkat cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: mouse brain tissue lysate（20 µg/Lane）
Lane 6: mouse liver tissue lysate（20 µg/Lane）

Lysates/proteins at 10 µg/Lane.

Predicted band size: 124 kDa
Observed band size: 110 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1606-36_2.jpg Fig2: ICC staining of PI3 Kinase p110α in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1606-36_3.jpg Fig3: ICC staining of PI3 Kinase p110α in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1606-36_4.jpg Fig4: ICC staining of PI3 Kinase p110α in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-36, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1606-36_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-36_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-36) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-36_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-PI3 Kinase p110α antibody (ET1606-36) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-36) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-36_8.jpg Fig8: PI3 Kinase p110α was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1606-36 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1606-36 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: ET1606-36 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of ET1606-36 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 2 minutes; ECL: K1802
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.