MMP-2 Recombinant Rabbit Monoclonal Antibody [SI15-04]
cat.: ET1606-4
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SI15-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MMP2 aa 101-150 / 660. |
| Positive control: | U-87 MG cell lysate, L6 cell lysate, mouse liver tissue. |
| Subcellular location: | Secreted, extracellular space, extracellular matrixm Membrane, Nucleus; Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P IP |
1:2,000 1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P08253 Human | P33434 Mouse | P33436 Rat |
| Alternative names: | 72 kDa gelatinase 72kD type IV collagenase CLG 4 CLG 4A CLG4 CLG4A Collagenase Type 4 alpha Collagenase type IV A Gelatinase A Gelatinase alpha Gelatinase neutrophil Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) Matrix Metalloproteinase 2 Matrix metalloproteinase II Matrix metalloproteinase-2 MMP 2 MMP II MMP-2 MMP2 MMP2_HUMAN MONA Neutrophil gelatinase PEX TBE 1 TBE-1 |
Images
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Fig1:
Western blot analysis of MMP-2 on different lysates with Rabbit anti-MMP-2 antibody (ET1606-4) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: L6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 74 kDa Observed band size: 70 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MMP-2 antibody (ET1606-4) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-4) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
MMP-2 was immunoprecipitated from 0.2 mg U-87 MG cell lysate with ET1606-4 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using ET1606-4 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: U-87 MG cell lysate (input) Lane 2: ET1606-4 IP in U-87 MG cell lysate Lane 3: Rabbit IgG instead of ET1606-4 in U-87 MG cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 59 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.