PD1 Recombinant Rabbit Monoclonal Antibody [SJ01-91]
cat.: ET1606-41
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SJ01-91
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 32 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human PD1 aa 1-50 / 288.
Positive control: over-expressed PD1(whole extracellular domain) on 293T cell lysates, human lymph nodes tissue.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: Q15116 Human
Alternative names: CD279 CD279 antigen hPD 1 hPD l hPD-1 hSLE1 PD 1 PD-1 PD1 PDCD 1 PDCD1 PDCD1_HUMAN Programmed cell death 1 Programmed cell death 1 protein Programmed cell death protein 1 Protein PD 1 Protein PD-1 SLEB2 Systemic lupus erythematosus susceptibility 2
Images
ET1606-41_1.jpg Fig1: Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PD1 antibody (ET1606-41) at 1/50 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-41) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.