Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09]
cat.: ET1606-49
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SJ19-09 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Integrin beta 3 aa 729-788 / 788. |
| Positive control: | U-87 MG cell lysates, HepG2, human spleen tissue, mouse testis tissue, human liver carcinoma tissue, human breast carcinoma tissue, mouse colon tissue, U87-MG-si-NT cell lysate. |
| Subcellular location: | Cell membrane, Cell projection, Cell junction. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:2,000 1:400-1:1,000 1:200 |
| Uniprot #: | SwissProt: P05106 Human | O54890 Mouse Entrez Gene: 29302 Rat |
| Alternative names: | BDPLT16 BDPLT2 CD 61 CD61 CD61 antigen GP3A GPIIIa GT HPA 1 HPA 4 Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) Integrin beta chain beta 3 Integrin beta-3 ITB3_HUMAN ITG B3 ITGB 3 ITGB3 NAIT Platelet fibrinogen receptor beta subunit Platelet fibrinogen receptor, beta subunit Platelet glycoprotein IIIa Platelet glycoprotein IIIa precursor Platelet membrane glycoprotein IIIa PTP |
Images
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Fig1:
Western blot analysis of Integrin beta 3 on U-87 MG cell lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 87 kDa Observed band size: 100 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/500 dilution. Lane 1: U87-MG-si NT cell lysate Lane 2: U87-MG-si Integrin beta 3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 87 kDa Observed band size: 87,100 kDa Exposure time: 1 minutes 10 seconds; 4-20% SDS-PAGE gel. ET1606-49 was shown to specifically react with Integrin beta 3 in U87-MG-si NT cells. Weakened band was observed when U87-MG-si Integrin beta 3 sample was tested. U87-MG-si NT and U87-MG-si Integrin beta 3 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1606-49, 1/500) and Loading control antibody (Rabbit anti-Hsp90, ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-49) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Integrin beta 3 antibody (ET1606-49) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-49) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.