CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]
cat.: ET1606-50
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IP
Clonality: Monoclonal
Clone number: SJ20-00
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD86 aa 1-50 / 329.
Positive control: Daudi cell lysate, Raji cell lysate, Ramos cell lysate, K-562 cell lysate, Mouse lung tissue lysate, Rat lung tissue lysate, Raji.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IP
1:5,000-1:20,000
1:500
Use at an assay dependent concentration.
Uniprot #: SwissProt: P42081 Human | P42082 Mouse
Entrez Gene: 56822 Rat
Alternative names: Activation B7-2 antigen 3 Activation B7-2 antigen B-lymphocyte activation antigen B7-2 2 B-lymphocyte activation antigen B7-2 B7 2 B7 B7-2 B7.2 B70 B72 antigen BU63 CD28 antigen ligand 2 2 CD28 antigen ligand 2 Cd28l2 CD28LG2 CD86 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) CD86 antigen CD86 molecule CD86_HUMAN CLS1 CTLA-4 counter-receptor B7.2 2, 3 CTLA-4 counter-receptor B7.2 Early T-cell costimulatory molecule 1 ETC-1 FUN 1 FUN-1 LAB72 Ly-58 Ly58 MB7 MB7-2 Membrane glycoprotein MGC34413 T-lymphocyte activation antigen CD86 TS/A-2
Images
ET1606-50_1.jpg Fig1: Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/5,000 dilution.

Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate
Lane 3: Ramos cell lysate
Lane 4: K-562 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes 37 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
ET1606-50_2.jpg Fig2: Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/5,000 dilution.

Lane 1: Raji cell lysate (10 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)
Lane 3: Rat lung tissue lysate (20 µg/Lane)

Predicted band size: 38 kDa
Observed band size: 70 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
ET1606-50_3.jpg Fig3: Immunocytochemistry analysis of Raji cells labeling CD86 with Rabbit anti-CD86 antibody (ET1606-50) at 1/500 dilution.

Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD86 antibody (ET1606-50) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.