Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IP, FC, IF-Cell |
Clonality: | Monoclonal |
Clone number: | SJ20-00 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 38 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human CD86 aa 1-50 / 329. |
Positive control: | Daudi cell lysate, Raji cell lysate, Ramos cell lysate, Jurkat cell lysate, K-562 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, Raji, K562. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB FC IP IF-Cell |
1:1,000-1:2,000 1:50-1:100 Use at an assay dependent concentration. 1:500 |
Uniprot #: | SwissProt: P42081 Human | P42082 Mouse Entrez Gene: 56822 Rat |
Alternative names: | Activation B7-2 antigen 3 Activation B7-2 antigen B-lymphocyte activation antigen B7-2 2 B-lymphocyte activation antigen B7-2 B7 2 B7 B7-2 B7.2 B70 B72 antigen BU63 CD28 antigen ligand 2 2 CD28 antigen ligand 2 Cd28l2 CD28LG2 CD86 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) CD86 antigen CD86 molecule CD86_HUMAN CLS1 CTLA-4 counter-receptor B7.2 2, 3 CTLA-4 counter-receptor B7.2 Early T-cell costimulatory molecule 1 ETC-1 FUN 1 FUN-1 LAB72 Ly-58 Ly58 MB7 MB7-2 Membrane glycoprotein MGC34413 T-lymphocyte activation antigen CD86 TS/A-2 |
Fig1:
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/2,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lane 4: Jurkat cell lysate Lane 5: K-562 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 38 kDa Observed band size: 70 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/1,000 dilution. Lane 1: Raji cell lysate (10 µg/Lane) Lane 2: Mouse lung tissue lysate (20 µg/Lane) Lane 3: Rat lung tissue lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 70 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Fig3: Flow cytometric analysis of CD86 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-50, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red). | |
Fig4: Flow cytometric analysis of CD86 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |