CD86 Recombinant Rabbit Monoclonal Antibody [SJ20-00]
cat.: ET1606-50
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IP, FC, IF-Cell
Clonality: Monoclonal
Clone number: SJ20-00
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CD86 aa 1-50 / 329.
Positive control: Daudi cell lysate, Raji cell lysate, Ramos cell lysate, Jurkat cell lysate, K-562 cell lysate, mouse lung tissue lysate, rat lung tissue lysate, Raji, K562.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  FC
  IP
  IF-Cell

1:1,000-1:2,000
1:50-1:100
Use at an assay dependent concentration.
1:500
Uniprot #: SwissProt: P42081 Human | P42082 Mouse
Entrez Gene: 56822 Rat
Alternative names: Activation B7-2 antigen 3 Activation B7-2 antigen B-lymphocyte activation antigen B7-2 2 B-lymphocyte activation antigen B7-2 B7 2 B7 B7-2 B7.2 B70 B72 antigen BU63 CD28 antigen ligand 2 2 CD28 antigen ligand 2 Cd28l2 CD28LG2 CD86 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) 1, 2 CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) CD86 antigen CD86 molecule CD86_HUMAN CLS1 CTLA-4 counter-receptor B7.2 2, 3 CTLA-4 counter-receptor B7.2 Early T-cell costimulatory molecule 1 ETC-1 FUN 1 FUN-1 LAB72 Ly-58 Ly58 MB7 MB7-2 Membrane glycoprotein MGC34413 T-lymphocyte activation antigen CD86 TS/A-2
Images
ET1606-50_1.jpg Fig1: Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/2,000 dilution.

Lane 1: Daudi cell lysate
Lane 2: Raji cell lysate
Lane 3: Ramos cell lysate
Lane 4: Jurkat cell lysate
Lane 5: K-562 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes 37 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1606-50_2.jpg Fig2: Western blot analysis of CD86 on different lysates with Rabbit anti-CD86 antibody (ET1606-50) at 1/1,000 dilution.

Lane 1: Raji cell lysate (10 µg/Lane)
Lane 2: Mouse lung tissue lysate (20 µg/Lane)
Lane 3: Rat lung tissue lysate (20 µg/Lane)

Predicted band size: 38 kDa
Observed band size: 70 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-50) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ET1606-50_3.jpg Fig3: Flow cytometric analysis of CD86 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-50, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
ET1606-50_4.jpg Fig4: Flow cytometric analysis of CD86 was done on Raji cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.