Musashi 1 Recombinant Rabbit Monoclonal Antibody [SJ201]
cat.: ET1606-51
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Pig, Cynomolgus monkey |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SJ201 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human Musashi 1. |
| Positive control: | SH-SY5Y cell lysate, Neuro-2a cell lysate, mouse P0 brain cell lysate, mouse cerebellum tissue, rat cerebellum tissue, Hela cell. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue FC IP |
1:2,000 1:200-1:500 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O43347 Human |
| Alternative names: | Msi 1 Msi1 MSI1H_HUMAN Musashi homolog 1 Musashi-1 Musashi1 RNA binding protein Musashi homolog 1 RNA-binding protein Musashi homolog 1 |
Images
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Fig1:
Western blot analysis of Musashi 1 on different lysates with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: mouse P0 brain cell lysate (20 µg/Lane) Predicted band size: 39 kDa Observed band size: 39 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-51) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Musashi 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-51, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.