Musashi 1 Recombinant Rabbit Monoclonal Antibody [SJ201]
cat.: ET1606-51
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, IHC-Fr
Clonality: Monoclonal
Clone number: SJ201
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 39 kDa
Isotype: IgG
Immunogen: Synthetic peptide within N-terminal human Musashi 1.
Positive control: Hela, A549, human pancreas tissue.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  IHC-Fr
  IF-Cell
  IF-Tissue
  FC
  IP
1:2000
1:200-1:500
1:500
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: O43347 Human
Alternative names: Msi 1 Msi1 MSI1H_HUMAN Musashi homolog 1 Musashi-1 Musashi1 RNA binding protein Musashi homolog 1 RNA-binding protein Musashi homolog 1
Images
ET1606-51_1.jpg Fig1: Western blot analysis of Musashi 1 on different lysates with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/2,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: mouse P0 brain cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-51) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1606-51_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-51_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-51_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-51) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-51_5.jpg Fig5: Immunofluorescence analysis of frozen mouse cerebellum tissue with Rabbit anti-Musashi 1 antibody (ET1606-51) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1606-51, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
ET1606-51_6.jpg Fig6: Flow cytometric analysis of Musashi 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-51, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.