SUMO-1 Recombinant Rabbit Monoclonal Antibody [SJ20-03]
cat.: ET1606-53
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC, ChIP
Clonality: Monoclonal
Clone number: SJ20-03
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 12 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Human SUMO-1 aa 51-100 / 101.
Positive control: HeLa cell lysate, A431 cell lysate, MCF7 cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Hela, A549, MCF-7, human tonsil tissue, human thyroid tissue, human breast carcinoma tissue, mouse thyroid tissue, mouse trachea tissue, mouse esophagus tissue.
Subcellular location: Nucleus membrane, Cytoplasm, Nucleus, Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
  ChIP
1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Use 0.5~2 μg for 25 μg of chromatin.
Uniprot #: SwissProt: P63165 Human | P63166 Mouse | Q5I0H3 Rat
Alternative names: DAP1 GAP modifying protein 1 GAP-modifying protein 1 GMP 1 GMP1 OFC10 PIC 1 PIC1 SENP2 Sentrin 1 Sentrin Small ubiquitin related modifier 1 Small ubiquitin-like modifier 1 Small ubiquitin-related modifier 1 SMT3 SMT3 homolog 3 SMT3 suppressor of mif two 3 homolog 1 SMT3, yeast, homolog 3 Smt3C SMT3H3 SUMO-1 SUMO1 SUMO1_HUMAN Ubiquitin homology domain protein PIC1 Ubiquitin Like 1 Ubiquitin like protein SMT3C Ubiquitin like protein UBL1 Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein SMT3C Ubiquitin-like protein UBL1 UBL 1 UBL1
Images
ET1606-53_1.jpg Fig1: Western blot analysis of SUMO-1 on different lysates with Rabbit anti-SUMO-1 antibody (ET1606-53) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 12 kDa
Observed band size: 17/80 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1606-53_2.jpg Fig2: ICC staining of SUMO-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1606-53_3.jpg Fig3: ICC staining of SUMO-1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1606-53_4.jpg Fig4: ICC staining of SUMO-1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1606-53, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1606-53_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SUMO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-53_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-SUMO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-53_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-SUMO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-53_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-SUMO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-53, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-53_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse trachea tissue with Rabbit anti-SUMO-1 antibody (ET1606-53) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-53) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-53_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-SUMO-1 antibody (ET1606-53) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-53) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1606-53_11.jpg Fig11: Flow cytometric analysis of SUMO-1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1606-53, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
ET1606-53_12.jpg Fig12: Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either SUMO-1 (ET1606-53) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
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