Anti-Synaptophysin antibody [SJ26-85]
cat.: ET1606-56
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SJ26-85
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: PC-12, N2A, human brain lysate, human pancreas tissue, mouse brain tissue, mouse pancreas tissue.
Subcellular location: Cytoplasmic, Cell junction, synapse, synaptosome.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:1,000-1:5,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P08247 Human | Q62277 Mouse | P07825 Rat
Alternative names: Major synaptic vesicle protein p38 MRX96 MRXSYP Syn p38 Synaptophysin Syp SYPH SYPH_HUMAN SypI
Images
ET1606-56_1.jpg Fig1: Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution.
Positive control:
Lane 1: Human brain
Lane 2: PC-12
ET1606-56_2.jpg Fig2: ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1606-56_3.jpg Fig3: ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1606-56_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
ET1606-56_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
ET1606-56_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.