CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01]
cat.: ET1607-1
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | SY21-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 64 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CDC7 Kinase aa 1-50 / 574. |
| Positive control: | Jurkat cell lysate, Hela cell lysate, A431. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000-1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: O00311 Human | Q9Z0H0 Mouse Unigene: 20842 Rat |
| Alternative names: | Cdc 7 CDC7 CDC7 cell division cycle 7 CDC7 cell division cycle 7 like 1 CDC7 L1 Cdc7 like 1 CDC7 related kinase CDC7-related kinase CDC7_HUMAN CDC7L 1 CDC7L1 Cell division cycle 7 homolog Cell division cycle 7 kinase Cell division cycle 7 like protein 1 Cell division cycle 7 related protein kinase Cell division cycle 7-related protein kinase HsCDC 7 HsCdc7 Hsk 1 Hsk1 HuCDC 7 huCdc7 MGC117361 MGC126237 MGC126238 |
Images
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Fig1:
Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Jurkat cell lysate Lane 2: Hela cell lysate |
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Fig2: Flow cytometric analysis of CDC7 Kinase was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1607-1, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.