CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01]
cat.: ET1607-1
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Monoclonal
Clone number: SY21-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 64 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human CDC7 Kinase aa 1-50 / 574.
Positive control: Jurkat cell lysate, Hela cell lysate, A431.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  FC

1:1,000-1:5,000
1:50-1:100
Uniprot #: SwissProt: O00311 Human | Q9Z0H0 Mouse
Unigene: 20842 Rat
Alternative names: Cdc 7 CDC7 CDC7 cell division cycle 7 CDC7 cell division cycle 7 like 1 CDC7 L1 Cdc7 like 1 CDC7 related kinase CDC7-related kinase CDC7_HUMAN CDC7L 1 CDC7L1 Cell division cycle 7 homolog Cell division cycle 7 kinase Cell division cycle 7 like protein 1 Cell division cycle 7 related protein kinase Cell division cycle 7-related protein kinase HsCDC 7 HsCdc7 Hsk 1 Hsk1 HuCDC 7 huCdc7 MGC117361 MGC126237 MGC126238
Images
ET1607-1_1.jpg Fig1: Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate
ET1607-1_2.jpg Fig2: Flow cytometric analysis of CDC7 Kinase was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1607-1, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.