Caveolin-2 Recombinant Rabbit Monoclonal Antibody [SY22-05]
cat.: ET1607-15
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SY22-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Caveolin-2 aa 1-50 / 162. |
| Positive control: | HUVEC, Hela, human cervix uteri tissue, human uterus tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P51636 Human |
| Alternative names: | CAV CAV2 CAV2_HUMAN Caveolae protein 20 kD Caveolin 2 Caveolin 2 isoform a and b Caveolin-2 MGC12294 OTTHUMP00000025032 OTTHUMP00000195982 |
Images
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Fig1:
Western blot analysis of Caveolin-2 on different lysates with Rabbit anti-Caveolin-2 antibody (ET1607-15) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Caveolin-2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 120 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-15) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of caveolin-2 on HUVEC cell lysates using anti-caveolin-2 antibody at 1/1,000 dilution. |
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Fig3: ICC staining caveolin-2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human cervix uteri tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-caveolin-2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.