Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06]
cat.: ET1607-16
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SY22-06 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Caveolin-3 aa 1-125 / 151. |
| Positive control: | Rat heart tissue, human striated muscle tissue, HEK-293 cell lysate, Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat heart tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Golgi apparatus membrane, Cell membrane, Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P56539 Human | P51637 Mouse | P51638 Rat |
| Alternative names: | CAV3 CAV3_HUMAN Caveolin 3 Caveolin-3 LGMD1C LQT9 M-caveolin MGC126100 MGC126101 MGC126129 OTTHUMP00000115603 OTTHUMP00000207105 VIP 21 VIP21 |
Images
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Fig1:
Western blot analysis of Caveolin-3 on different lysates with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Rat heart tissue lysate Lane 5: Rat skeletal muscle tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Negative expression of Caveolin-3 protein in HEK-293 cell is consistent with the predicted expression pattern. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1607-16, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 17 kDa Observed band size: 17 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.