CD63 Recombinant Rabbit Monoclonal Antibody [SY21-02]
cat.: ET1607-2
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SY21-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD63 aa 88-216 / 238. |
| Positive control: | SK-MEL-28 cell lysate, U-87 MG cell lysate, human lung carcinoma tissue, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Lysosome membrane, Cell membrane, extracellular exosome, Late endosome membrane, multivesicular body, Melanosome, Cell surface. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P08962 Human |
| Alternative names: | Lysosomal associated membrane protein 3 CD 63 CD63 CD63 antigen (melanoma 1 antigen) CD63 antigen CD63 antigen melanoma 1 antigen CD63 molecule CD63_HUMAN gp55 Granulophysin LAMP 3 LAMP-3 LAMP3 LIMP Lysosomal-associated membrane protein 3 Lysosome associated membrane glycoprotein 3 Mast cell antigen AD1 ME491 Melanoma 1 antigen Melanoma associated antigen ME491 Melanoma associated antigen MLA1 Melanoma-associated antigen ME491 MGC72893 MLA 1 MLA1 NGA Ocular melanoma associated antigen Ocular melanoma-associated antigen OMA81H PTLGP40 Tetraspanin 30 Tetraspanin-30 Tspan 30 Tspan-30 TSPAN30 |
Images
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Fig1:
Western blot analysis of CD63 on different lysates with Rabbit anti-CD63 antibody (ET1607-2) at 1/2,000 dilution. Lane 1: SK-MEL-28 cell lysate Lane 2: U-87 MG cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 26 kDa Observed band size: 30~60 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD63 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-2, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Western blot analysis of CD63 on different lysates with Rabbit anti-CD63 antibody (ET1607-2) at 1/2,000 dilution. Lane 1: SK-MEL-28-si NT cell lysate Lane 2: SK-MEL-28-si CD63 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 30~60 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-2) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.