CD3 epsilon Recombinant Rabbit Monoclonal Antibody [SY0239]
cat.: ET1607-29
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SY0239 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD3 epsilon aa 153-203 (Cytoplasmic). |
| Positive control: | Human thymus tissue lysate, Jurkat cell lysate, human tonsil tissue, human spleen tissue, Jurkat. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell IF-Tissue |
1:500-1:2,000 1:50-1:1;000 1:50-1:100 1:200 |
| Uniprot #: | SwissProt: P07766 Human |
| Alternative names: | CD3 epsilon CD3e CD3e antigen epsilon polypeptide (TiT3 complex) CD3E antigen epsilon polypeptide CD3E antigen, epsilon subunit CD3e molecule epsilon CD3e molecule, epsilon (CD3 TCR complex) CD3e molecule, epsilon (CD3-TCR complex) CD3E_HUMAN IMD18 T cell antigen receptor complex epsilon subunit of T3 T cell surface antigen T3/Leu 4 epsilon chain T cell surface glycoprotein CD3 epsilon chain T-cell surface antigen T3/Leu-4 epsilon chain T-cell surface glycoprotein CD3 epsilon chain T3E TCRE |
Images
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Fig1:
Western blot analysis of CD3 epsilon on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human thymus tissue lysate Lane 2: Jurkat cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3 epsilon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-29, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Flow cytometric analysis of Jurkat cells labeling CD3 epsilon. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1607-29, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Immunocytochemistry analysis of Jurkat cells labeling CD3 epsilon with Rabbit anti-CD3 epsilon antibody (ET1607-29) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD3 epsilon antibody (ET1607-29) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 epsilon antibody (ET1607-29) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-29) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.