GFP Recombinant Rabbit Monoclonal Antibody [SY0243]
cat.: ET1607-31
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB, IF-Cell, IF-Tissue, IP, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SY0243 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238. |
| Positive control: | GFP recombinant protein. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IP IHC-P |
1:10,000 1:50-1:200 1:50-1:200 2-5 µg/ml. 1:5,000 |
| Uniprot #: | SwissProt: P42212 AequoreaVictoria |
| Alternative names: | GFP Green fluorescent protein yfp |
Images
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Fig1:
Western blot analysis of GFP on GFP recombinant protein with Rabbit anti-GFP antibody (ET1607-31) at 1/10,000 dilution. Lysates/proteins at 50 ng/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-31) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with ET1607-31 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: GFP Tag fusion protein lysate (input). Lane 2: Rabbit IgG instead of ET1607-31 in GFP Tag fusion protein lysate. Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate. Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate. Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate. Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate. Lane 7: R1312-2 IP in GFP Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST |
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Fig3:
Immunocytochemistry analysis of HeLa cells transfected with N-terminal GFP labeling GFP with Rabbit anti-GFP antibody (ET1607-31) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GFP antibody (ET1607-31) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with GFP (green). Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1607-31) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-31) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue transfected with GFP-tagged CX3CR1 with Rabbit anti-GFP antibody (ET1607-31) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-31) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.