JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]
cat.: ET1607-35
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SY0245 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 131 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human JAK2. |
| Positive control: | TF-1 cell lysate, K-562 cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Jurkat cell lysate, MEF cell lysate, A549, MCF-7, NIH/3T3. |
| Subcellular location: | Endomembrane system, Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: O60674 Human | Q62120 Mouse | Q62689 Rat |
| Alternative names: | JAK 2 JAK-2 JAK2 JAK2_HUMAN Janus Activating Kinase 2 Janus kinase 2 (a protein tyrosine kinase) Janus kinase 2 JTK 10 JTK10 kinase Jak2 OTTHUMP00000043260 THCYT3 Tyrosine protein kinase JAK2 Tyrosine-protein kinase JAK2 |
Images
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Fig1:
Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: K-562 cell lysate Lane 3: THP-1 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 131 kDa Observed band size: 120 kDa Exposure time: 5 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: MEF cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 131 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution. Lane 1: A549-si NT cell lysate Lane 2: A549-si JAK2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 131 kDa Observed band size: 120 kDa Exposure time: 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig4: ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.