JAK2 Recombinant Rabbit Monoclonal Antibody [SY0245]
cat.: ET1607-35
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: SY0245
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 131 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human JAK2.
Positive control: TF-1 cell lysate, K-562 cell lysate, THP-1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Jurkat cell lysate, MEF cell lysate, A549, MCF-7, NIH/3T3.
Subcellular location: Endomembrane system, Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell

1:500-1:2,000
1:100-1:500
Uniprot #: SwissProt: O60674 Human | Q62120 Mouse | Q62689 Rat
Alternative names: JAK 2 JAK-2 JAK2 JAK2_HUMAN Janus Activating Kinase 2 Janus kinase 2 (a protein tyrosine kinase) Janus kinase 2 JTK 10 JTK10 kinase Jak2 OTTHUMP00000043260 THCYT3 Tyrosine protein kinase JAK2 Tyrosine-protein kinase JAK2
Images
ET1607-35_1.jpg Fig1: Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution.

Lane 1: TF-1 cell lysate
Lane 2: K-562 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 131 kDa
Observed band size: 120 kDa

Exposure time: 5 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1607-35_2.png Fig2: Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/500 dilution.

Lane 1: Jurkat cell lysate
Lane 2: MEF cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 131 kDa
Observed band size: 120 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ET1607-35_3.jpg Fig3: Western blot analysis of JAK2 on different lysates with Rabbit anti-JAK2 antibody (ET1607-35) at 1/2,000 dilution.

Lane 1: A549-si NT cell lysate
Lane 2: A549-si JAK2 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 131 kDa
Observed band size: 120 kDa

Exposure time: 45 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
ET1607-35_4.jpg Fig4: ICC staining JAK2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1607-35_5.jpg Fig5: ICC staining JAK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1607-35_6.jpg Fig6: ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.