Anti-Smad1 antibody [SY0254]
cat.: ET1607-42
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC/IF
Clonality: Monoclonal
Clone number: SY0254
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 52 kDa
Isotype: IgG
Immunogen: recombinant protein
Positive control: PANC-1, MCF-7, Hela, A431.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  ICC/IF

1:500-1:1,000
1:100-1:500
Uniprot #: Q15797(Human) P70340(Mouse)
Alternative names: BSP-1 antibody BSP1 antibody HsMAD1 antibody JV4-1 antibody JV41 antibody MAD homolog 1 antibody MAD mothers against decapentaplegic homolog 1 antibody Mad related protein 1 antibody Mad-related protein 1 antibody MADH1 antibody MADR1 antibody Mothers against decapentaplegic homolog 1 antibody Mothers against DPP homolog 1 antibody SMA- AND MAD-RELATED PROTEIN 1 antibody SMAD 1 antibody SMAD family member 1 antibody SMAD mothers against DPP homolog 1 antibody Smad1 antibody SMAD1_HUMAN antibody TGF beta signaling protein 1 antibody Transforming growth factor-beta-signaling protein 1 antibody
Images
ET1607-42_1.jpg Fig1: ICC staining Smad1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1607-42_2.jpg Fig2: ICC staining Smad1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1607-42_3.jpg Fig3: ICC staining Smad1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ET1607-42_4.jpg Fig4: ICC staining Smad1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.