Phospho-p95/NBS1 (S343) Recombinant Rabbit Monoclonal Antibody [SY0215]
cat.: ET1607-5
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SY0215 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 85 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser343 of Human p95 / NBS1 aa 321-370 / 754. |
| Positive control: | HeLa treated with 20μM Etoposide for 2 hours whole cell lysate, Jurkat treated with 25μM Etoposide for 5 hours whole cell lysate, PC-3M, mouse testis tissue. |
| Subcellular location: | Nucleus, PML body, telomere, Chromosome. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O60934 Human | Q9R207 Mouse |
| Alternative names: | AT V1 AT V2 ATV Cell cycle regulatory protein p95 FLJ10155 MGC87362 Nbn NBN_HUMAN NBS 1 NBS NBS1 Nibrin Nijmegen breakage syndrome 1 (nibrin) Nijmegen breakage syndrome Nijmegen breakage syndrome protein 1 p95 p95 protein of the MRE11/RAD50 complex |
Images
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Fig1:
Western blot analysis of Phospho-p95/NBS1 (S343) on different lysates with Rabbit anti-Phospho-p95/NBS1 (S343) antibody (ET1607-5) at 1/1,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa treated with 20μM Etoposide for 2 hours whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Jurkattreated with 25μM Etoposide for 5 hours whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 95 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-5) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Phospho-p95/NBS1 (S343) in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-p95/NBS1 (S343) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.