Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Rat, Mouse |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | SY26-04 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 16 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human DNAJC15 aa 10-150 / 150. |
Positive control: | Human brain tissue lysates, mouse heart tissue lysates, human liver carcinoma tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue, mouse heart tissue. |
Subcellular location: | Mitochondrion inner membrane. |
Recommended Dilutions:
WB |
1:500-1:2,000 |
Uniprot #: | SwissProt: Q9Y5T4 Human | Q78YY6 Mouse Entrez Gene: 290370 Rat |
Alternative names: | Cell growth inhibiting gene 22 protein Cell growth-inhibiting gene 22 protein DJC15_HUMAN DnaJ (Hsp40) homolog subfamily C member 15 DNAJ domain containing DnaJ homolog subfamily C member 15 DNAJC15 DNAJD1 GIG22 HSD18 MCJ Methylation controlled J protein Methylation-controlled J protein |
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Fig1:
Western blot analysis of DNAJC15 on human brain tissue lysates with Rabbit anti-DNAJC15 antibody (ET1607-52) at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-52) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |