DNAJC15 Recombinant Rabbit Monoclonal Antibody [SY26-04]
cat.: ET1607-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB
Clonality: Monoclonal
Clone number: SY26-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 16 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human DNAJC15 aa 10-150 / 150.
Positive control: Human brain tissue lysates, mouse heart tissue lysates, human liver carcinoma tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue, mouse heart tissue.
Subcellular location: Mitochondrion inner membrane.
Recommended Dilutions:
  WB

1:500-1:2,000
Uniprot #: SwissProt: Q9Y5T4 Human | Q78YY6 Mouse
Entrez Gene: 290370 Rat
Alternative names: Cell growth inhibiting gene 22 protein Cell growth-inhibiting gene 22 protein DJC15_HUMAN DnaJ (Hsp40) homolog subfamily C member 15 DNAJ domain containing DnaJ homolog subfamily C member 15 DNAJC15 DNAJD1 GIG22 HSD18 MCJ Methylation controlled J protein Methylation-controlled J protein
Images
ET1607-52_1.jpg Fig1: Western blot analysis of DNAJC15 on human brain tissue lysates with Rabbit anti-DNAJC15 antibody (ET1607-52) at 1/2,000 dilution.

Lysates/proteins at 40 µg/Lane.

Predicted band size: 16 kDa
Observed band size: 16 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-52) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.