DNAJC15 Recombinant Rabbit Monoclonal Antibody [SY26-04]
cat.: ET1607-52
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Monoclonal
Clone number: SY26-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 16 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human DNAJC15 aa 10-150 / 150.
Positive control: Human brain tissue lysates, mouse heart tissue lysates, human liver carcinoma tissue, human kidney tissue, mouse testis tissue, mouse kidney tissue, mouse heart tissue.
Subcellular location: Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:2,000
1:50-1:200
Uniprot #: SwissProt: Q9Y5T4 Human | Q78YY6 Mouse
Entrez Gene: 290370 Rat
Alternative names: Cell growth inhibiting gene 22 protein Cell growth-inhibiting gene 22 protein DJC15_HUMAN DnaJ (Hsp40) homolog subfamily C member 15 DNAJ domain containing DnaJ homolog subfamily C member 15 DNAJC15 DNAJD1 GIG22 HSD18 MCJ Methylation controlled J protein Methylation-controlled J protein
Images
ET1607-52_1.jpg Fig1: Western blot analysis of DNAJC15 on human brain tissue lysates with Rabbit anti-DNAJC15 antibody (ET1607-52) at 1/2,000 dilution.

Lysates/proteins at 40 µg/Lane.

Predicted band size: 16 kDa
Observed band size: 16 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-52) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1607-52_2.jpg Fig2: Western blot analysis of DNAJC15 on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-52, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1607-52_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-DNAJC15 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1607-52_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DNAJC15 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1607-52_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-DNAJC15 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1607-52_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DNAJC15 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-52, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1607-52_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-DNAJC15 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-52, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.