Anti-MKLP1 antibody [SY02-74]
cat.: ET1607-63
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, ICC/IF |
| Clonality: | Monoclonal |
| Clone number: | SY02-74 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MKLP1 aa 800-840. |
| Positive control: | 293 cell lysate, A549 cell lysate, Hela, A549. |
| Subcellular location: | Cytoplasm, Nucleus, Midbody. |
| Recommended Dilutions:
WB ICC/IF |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q02241 Human Unigene: 259374 Mouse | 63734 Rat |
| Alternative names: | CHO 1 CHO1 KIF 23 KIF23 KIF23_HUMAN Kinesin family member 23 Kinesin like 5 Kinesin like protein 5 Kinesin like protein KIF 23 Kinesin like protein KIF23 Kinesin-like protein 5 Kinesin-like protein KIF23 KNS L5 KNSL 5 KNSL5 Mitotic kinesin like 1 Mitotic kinesin like protein 1 Mitotic kinesin-like protein 1 MKLP 1 |
Images
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Fig1:
Western blot analysis of MKLP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: A549 cell lysate |
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Fig2: ICC staining of MKLP1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: ICC staining of MKLP1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.