Anti-MKLP1 antibody [SY02-74]
cat.: ET1607-63
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF
Clonality: Monoclonal
Clone number: SY02-74
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 110 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MKLP1 aa 800-840.
Positive control: 293 cell lysate, A549 cell lysate, Hela, A549.
Subcellular location: Cytoplasm, Nucleus, Midbody.
Recommended Dilutions:
  WB
  ICC/IF

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: Q02241 Human
Unigene: 259374 Mouse | 63734 Rat
Alternative names: CHO 1 CHO1 KIF 23 KIF23 KIF23_HUMAN Kinesin family member 23 Kinesin like 5 Kinesin like protein 5 Kinesin like protein KIF 23 Kinesin like protein KIF23 Kinesin-like protein 5 Kinesin-like protein KIF23 KNS L5 KNSL 5 KNSL5 Mitotic kinesin like 1 Mitotic kinesin like protein 1 Mitotic kinesin-like protein 1 MKLP 1
Images
ET1607-63_1.jpg Fig1: Western blot analysis of MKLP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: A549 cell lysate
ET1607-63_2.jpg Fig2: ICC staining of MKLP1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1607-63_3.jpg Fig3: ICC staining of MKLP1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-63, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.