MKLP1 Recombinant Rabbit Monoclonal Antibody [SY02-74]
cat.: ET1607-63
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SY02-74 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MKLP1 aa 801-850 / 960. |
| Positive control: | A549 cell lysates, RAW264.7 cell lysates, A549. |
| Subcellular location: | Cytoplasm, Nucleus, Midbody. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q02241 Human Unigene: 259374 Mouse | 63734 Rat |
| Alternative names: | CHO 1 CHO1 KIF 23 KIF23 KIF23_HUMAN Kinesin family member 23 Kinesin like 5 Kinesin like protein 5 Kinesin like protein KIF 23 Kinesin like protein KIF23 Kinesin-like protein 5 Kinesin-like protein KIF23 KNS L5 KNSL 5 KNSL5 Mitotic kinesin like 1 Mitotic kinesin like protein 1 Mitotic kinesin-like protein 1 MKLP 1 |
Images
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Fig1:
Western blot analysis of MKLP1 on A549 cell lysates with Rabbit anti-MKLP1 antibody (ET1607-63) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-63) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of MKLP1 on RAW264.7 cell lysates with Rabbit anti-MKLP1 antibody (ET1607-63) at 1/500 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 110 kDa Observed band size: 100/110 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-63) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of A549 cells labeling MKLP1 with Rabbit anti-MKLP1 antibody (ET1607-63) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MKLP1 antibody (ET1607-63) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.