PI 3 Kinase Class 3 Recombinant Rabbit Monoclonal Antibody [SY0286]
cat.: ET1607-74
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | SY0286 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 102 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PI 3 Kinase Class 3 aa 830-870. |
| Positive control: | Human stomach cancer tissue, mouse brain tissue, rat brain tissue, A549. |
| Subcellular location: | Midbody, Late endosome, Cytoplasmic vesicle. |
| Recommended Dilutions:
IHC-P FC |
1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q8NEB9 Human | Q6PF93 Mouse | O88763 Rat |
| Alternative names: | hVps34 MGC61518 Phosphatidylinositol 3 kinase catalytic subunit type 3 Phosphatidylinositol 3 kinase class 3 Phosphatidylinositol 3 kinase p100 subunit Phosphatidylinositol 3-kinase catalytic subunit type 3 Phosphatidylinositol 3-kinase p100 subunit Phosphoinositide 3 kinase class 3 Phosphoinositide-3-kinase class 3 PI3 kinase type 3 PI3-kinase type 3 PI3K type 3 Pik3c3 PK3C3_HUMAN PtdIns 3 kinase type 3 PtdIns-3-kinase type 3 Vps 34 Vps34 |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue with Rabbit anti-PI 3 Kinase Class 3 antibody (ET1607-74) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PI 3 Kinase Class 3 antibody (ET1607-74) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase Class 3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-74, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Flow cytometric analysis of A549 cells labeling PI 3 Kinase Class 3. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1607-74, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.