Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, ICC/IF, IHC-P, IP, FC |
Clonality: | Monoclonal |
Clone number: | SY29-02 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1 mg/mL. |
Purification: | Protein A affinity purified. |
Molecular weight: | 55 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within C-terminal human HDAC2. |
Positive control: | 293T cell lysate, K562 cell lysate, Hela, SHG-44, 293, rat spinal cord tissue, human tonsil tissue, human breast carcinoma tissue, mouse spinal cord tissue. |
Subcellular location: | Nucleus, Cytoplasm. |
Recommended Dilutions:
WB ICC/IF IHC-P FC IP |
1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
Uniprot #: | SwissProt: Q92769 Human | P70288 Mouse Unigene: 1797 Rat |
Alternative names: | D10Wsu179e antibody HD 2 antibody HD2 antibody HDAC 2 antibody Hdac2 antibody HDAC2_HUMAN antibody Histone deacetylase 2 (HD2) antibody Histone deacetylase 2 antibody OTTHUMP00000017046 antibody OTTHUMP00000227077 antibody OTTHUMP00000227078 antibody RPD3 antibody transcriptional regulator homolog RPD3 antibody YAF1 antibody YY1 associated factor 1 antibody YY1 transcription factor binding protein antibody Yy1bp antibody |
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Fig1:
Western blot analysis of HDAC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293T cell lysate Lane 2: K562 cell lysate |
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Fig2: ICC staining of HDAC2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of HDAC2 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of HDAC2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-HDAC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-HDAC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-HDAC2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-78, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9: Flow cytometric analysis of HDAC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1607-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |