Plectin Recombinant Rabbit Monoclonal Antibody [SY29-04]
cat.: ET1607-80
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP, IF-Tissue, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SY29-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 532 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Plectin aa 2-101 / 4,684. |
| Positive control: | Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa. |
| Subcellular location: | Cell junction, Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P IP IF-Tissue IF-Cell |
1:1,000 1:200-1:1,000 1-2μg/sample 1:100-1:200 1:100 |
| Uniprot #: | SwissProt: Q15149 Human | Q9QXS1 Mouse | P30427 Rat |
| Alternative names: | EBS1 EBSO HD1 Hemidesmosomal protein 1 PCN pleC PLEC_HUMAN PLEC1 PLEC1b Plectin 1 Plectin 1 intermediate filament binding protein 500kDa Plectin 6 Plectin Plectin-1 PLTN |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-Plectin antibody (ET1607-80) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-80) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-Plectin antibody (ET1607-80) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-80) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-Plectin antibody (ET1607-80) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-80) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunocytochemistry analysis of HeLa cells labeling Plectin with Rabbit anti-Plectin antibody (ET1607-80) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Plectin antibody (ET1607-80) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Plectin was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1607-80 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1607-80 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1607-80 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1607-80 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.