Bim Recombinant Rabbit Monoclonal Antibody [SU0318]
cat.: ET1608-14
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SU0318 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Bim aa 1-40. |
| Positive control: | Raji cell lysate, HeLa cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, Hela, A431, HepG2, human kidney tissue, RAW264.7, MCF7. |
| Subcellular location: | Endomembrane system, Mitochondrion. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP FC |
1:5,000 1:50-1:200 1:50-1:200 Use at an assay dependent concentration. 1:1,000 |
| Uniprot #: | SwissProt: O43521 Human | O54918 Mouse |
| Alternative names: | BCL2 like 11 B2L11_HUMAN BAM Bcl 2 interacting protein Bim Bcl 2 related ovarian death agonist Bcl-2-like protein 11 BCL2 interacting mediator of cell death BCL2 like 11 (apoptosis facilitator) BCL2 like protein 11 Bcl2-interacting mediator of cell death Bcl2-L-11 Bcl2l11 BIM alpha6 BIM BIM beta6 BIM beta7 BimEL BimL BOD |
Images
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Fig1:
Western blot analysis of Bim on different lysates with Rabbit anti-Bim antibody (ET1608-14) at 1/5,000 dilution. Lane 1: Raji cell lysate Lane 2: HeLa cell lysate Lane 3: MCF7 cell lysate Lane 4: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 22 kDa Observed band size: 25 kDa Exposure time: 3 minutes 49 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-14) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of Bim in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-14, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of Bim in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-14, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: ICC staining of Bim in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-14, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig5:
Application: Immunohistochemistry (IHC-P) Species: Human Tissue: Kidney Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1608-14, 1/200, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig6:
Flow cytometric analysis of RAW264.7 cells labeling Bim. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-14, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig7:
Flow cytometric analysis of MCF7 cells labeling Bim. Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-14, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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