Anti-Caldesmon antibody [SU03-19]
cat.: ET1608-16
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SU03-19
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 93 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human Caldesmon.
Positive control: NIH/3T3 cell lysates, NIH/3T3, C2C12, human kidney tissue, human uterus tissue, mouse kidney tissue.
Subcellular location: Cytoskeleton, stress fiber, myofibril.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P
  FC
  IP

1:1,000-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Use at an assay dependent concentration.
Uniprot #: SwissProt: Q05682 Human | Q62736 Rat
Unigene: 308134 Mouse
Alternative names: CAD antibody CALD 1 antibody CALD1 antibody CALD1_HUMAN antibody Caldesmon 1 antibody Caldesmon 1 Isoform 1 antibody Caldesmon 1 Isoform 2 antibody Caldesmon 1 Isoform 3 antibody Caldesmon 1 Isoform 4 antibody Caldesmon 1 Isoform 5 antibody Caldesmon antibody Caldesmon1 antibody CDM antibody H CAD antibody HCAD antibody L CAD antibody LCAD antibody MGC21352 antibody NAG22 antibody
Images
ET1608-16_1.jpg Fig1: Western blot analysis of Caldesmon on NIH/3T3 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-16, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ET1608-16_2.jpg Fig2: ICC staining of Caldesmon in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-16_3.jpg Fig3: ICC staining of Caldesmon in C2C12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-16, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ET1608-16_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-16_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-16_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-16, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-16_7.jpg Fig7: Flow cytometric analysis of Caldesmon was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-16, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.