LGR5/GPR49 Recombinant Rabbit Monoclonal Antibody [SU32-04]
cat.: ET1608-18
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | SU32-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LGR5 aa 816-865 / 907. |
| Positive control: | Mouse spinal cord tissue lysate, human skeletal muscle tissue lysate, SH-SY5Y, rat skeletal muscle tissue lysate, rat kidney tissue lysate, rat spinal cord tissue lysate, Mouse skeletal muscle tissue lysate. |
| Subcellular location: | Cell membrane, Golgi apparatus, Membrane. |
| Recommended Dilutions:
WB FC IP |
1:5,000 1:50 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O75473 Human | Q9Z1P4 Mouse Unigene: 214063 Rat |
| Alternative names: | FEX G protein coupled receptor 49 G protein coupled receptor 67 g protein-coupled receptor fex G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 GPR 49 GPR 67 GPR49 GPR67 GRP 49 GRP49 HG 38 HG38 Leucine rich repeat containing G protein coupled receptor 5 Leucine-rich repeat-containing G-protein coupled receptor 5 LGR 5 LGR5 LGR5_HUMAN MGC117008 Orphan G protein coupled receptor HG38 |
Images
|
Fig1:
Western blot analysis of LGR5/GPR49 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spinal cord tissue lysate Lane 2: Human skeletal muscle tissue lysate Predicted band size: 100 kDa Observed band size: 100 kDa |
|
Fig2:
Western blot analysis of LGR5/GPR49 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat skeletal muscle tissue lysate Lane 2: Rat kidney tissue lysate Lane 3: Rat spinal cord tissue lysate Predicted band size: 100 kDa Observed band size: 100 kDa |
|
Fig3:
Western blot analysis of LGR5/GPR49 on different lysates with Rabbit anti-LGR5/GPR49 antibody (ET1608-18) at 1/5,000 dilution. Lane 1: Mouse skeletal muscle tissue lysate Lane 2: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-18) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig4: Flow cytometric analysis of LGR5/GPR49 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-18, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.