Anti-LGR5/GPR49 antibody [SU32-04]
cat.: ET1608-18
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IP
Clonality: Monoclonal
Clone number: SU32-04
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 100 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human LGR5 aa 810-860.
Positive control: Mouse spinal cord tissue lysate, human skeletal muscle tissue lysate, mouse skeletal muscle tissue, SH-SY5Y.
Subcellular location: trans-Golgi network membrane, Cell membrane.
Recommended Dilutions:

Use at an assay dependent concentration.
Uniprot #: SwissProt: O75473 Human | Q9Z1P4 Mouse
Unigene: 214063 Rat
Alternative names: FEX G protein coupled receptor 49 G protein coupled receptor 67 g protein-coupled receptor fex G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 GPR 49 GPR 67 GPR49 GPR67 GRP 49 GRP49 HG 38 HG38 Leucine rich repeat containing G protein coupled receptor 5 Leucine-rich repeat-containing G-protein coupled receptor 5 LGR 5 LGR5 LGR5_HUMAN MGC117008 Orphan G protein coupled receptor HG38
ET1608-18_1.jpg Fig1: Western blot analysis of LGR5/GPR49 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-18, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse spinal cord tissue lysate
Lane 2: human skeletal muscle tissue lysate
ET1608-18_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-LGR5/GPR49 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-18_3.jpg Fig3: Flow cytometric analysis of LGR5/GPR49 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-18, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).