ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26]
cat.: ET1608-20
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | SU03-26 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 76 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ABCG1 aa 161-210 / 678. |
| Positive control: | THP-1 cell lysate, MCF-7 cell lysate, human lung tissue, human skin tissue. |
| Subcellular location: | Endoplasmic reticulum membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P45844 Human | Q64343 Mouse | A6JJX9 Rat |
| Alternative names: | ABC transporter 8 ABC8 ABCG1 ABCG1_HUMAN ATP-binding cassette sub family G member 1 ATP-binding cassette sub-family G member 1 ATP-binding cassette transporter 8 ATP-binding cassette transporter member 1 of subfamily G ATP-binding cassette, sub family G WHITE member 1 homolog of Drosophila white MGC34313 White protein homolog White protein homolog ATP binding cassette transporter 8 WHITE1 WHT1 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Western blot analysis of ABCG1 on MCF7 cell lysate with Rabbit anti-ABCG1 antibody (ET1608-20) at 1/2000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1608-20, 1/2000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 75.6 kDa Observed band size: 100 kDa |
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Fig4:
Western blot analysis of ABCG1 on THP-1 cell lysate with Rabbit anti-ABCG1 antibody (ET1608-20) at 1/2000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ET1608-20, 1/2000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 75.6 kDa Observed band size: 100 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.