Anti-ABCG1 antibody [SU03-26]
cat.: ET1608-20
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, ICC/IF, IHC-P
Clonality: Monoclonal
Clone number: SU03-26
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 110 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human ABCG1 aa 180-220.
Positive control: THP-1 cell lysate, MCF-7 cell lysate, human lung tissue, human skin tissue.
Subcellular location: Endoplasmic reticulum membrane, Golgi apparatus membrane.
Recommended Dilutions:
  WB
  ICC/IF
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P45844 Human
Unigene: 8398 Rat
Alternative names: ABC transporter 8 antibody ABC8 antibody ABCG1 antibody ABCG1_HUMAN antibody ATP-binding cassette sub family G member 1 antibody ATP-binding cassette sub-family G member 1 antibody ATP-binding cassette transporter 8 antibody ATP-binding cassette transporter member 1 of subfamily G antibody ATP-binding cassette, sub family G WHITE member 1 antibody homolog of Drosophila white antibody MGC34313 antibody White protein homolog antibody White protein homolog ATP binding cassette transporter 8 antibody WHITE1 antibody WHT1 antibody
Images
ET1608-20_1.jpg Fig1: Western blot analysis of ABCG1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: THP-1 cell lysate
Lane 2: MCF-7 cell lysate
ET1608-20_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-20_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.