Bak Recombinant Rabbit Monoclonal Antibody [SU32-07]
cat.: ET1608-21
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Tissue, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SU32-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Bak aa 1-50 / 211. |
| Positive control: | HeLa cell lysate, AGS cell lysate, HEK-293 cell lysate, human colon tissue, human stomach carcinoma tissue, human small intestine tissue. |
| Subcellular location: | Mitochondrion membrane. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IP |
1:1,000-1:2,000 1:50-1:200 1:500-1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q16611 Human |
| Alternative names: | Apoptosis regulator BAK BAK BAK like Bak NT BAK_HUMAN Bak1 Bcl 2 homologous antagonist/killer Bcl 2 like 7 protein Bcl-2 homologous antagonist/killer Bcl-2-like protein 7 BCL2 antagonist/killer 1 Bcl2 like 7 Protein Bcl2-L-7 BCL2L7 CDN1 Cell death inhibitor 1 MGC117255 MGC3887 NBak Pro apoptotic protein BAK |
Images
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Fig1:
Western blot analysis of Bak on different lysates with Rabbit anti-Bak antibody (ET1608-21) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: AGS cell lysate Lane 3: HEK-293 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Bak on different lysates with Rabbit anti-Bak antibody (ET1608-21) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Bak KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-21) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Bak antibody (ET1608-21) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-21) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with Rabbit anti-Bak antibody (ET1608-21) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-21) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Bak antibody (ET1608-21) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-21) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6:
Bak was immunoprecipitated from 0.2 mg HeLa cell lysate with ET1608-21 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using ET1608-21 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: ET1608-21 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of ET1608-21 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.