Phospho-IRF3 (S386) Recombinant Rabbit Monoclonal Antibody [SU03-28]
cat.: ET1608-22
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SU03-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser386 of human IRF3. |
| Positive control: | MCF7 treated with 100nM Calyculin A for 30 minutes whole cell lysate, NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, MCF-7. |
| Subcellular location: | Cytoplasm, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue |
1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q14653 Human | P70671 Mouse |
| Alternative names: | IIAE7 Interferon regulatory factor 3 IRF 3 IRF-3 IRF3 IRF3_HUMAN MGC94729 |
Images
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Fig1:
Western blot analysis of Phospho-IRF3 (S386) on different lysates with Rabbit anti-Phospho-IRF3 (S386) antibody (ET1608-22) at 1/1,000 dilution. Lane 1: MCF7 whole cell lysate Lane 2: MCF7 treated with 100nM Calyculin A for 30 minutes whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-22) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Phospho-IRF3 (S386) on different lysates with Rabbit anti-Phospho-IRF3 (S386) antibody (ET1608-22) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate Lane 3: NIH/3T3 treated with 100nM Calyculin A for 30 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining of Phospho-IRF3 (S386) in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-22, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.