DARPP32 Recombinant Rabbit Monoclonal Antibody [SU0329]
cat.: ET1608-23
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IP, FC |
| Clonality: | Monoclonal |
| Clone number: | SU0329 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DARPP32 aa 1-94 / 204. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, SH-SY5Y, MCF-7, Hela. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:5,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UD71 Human | Q60829 Mouse | Q6J4I0 Rat |
| Alternative names: | DARPP 32 DARPP-32 Dopamine and cAMP regulated neuronal phosphoprotein 32 Dopamine and cAMP regulated neuronal phosphoprotein Dopamine and cAMP regulated phosphoprotein Dopamine and cAMP regulated phosphoprotein DARPP 32 Dopamine and cAMP regulated phosphoprotein DARPP32 Dopamine- and cAMP-regulated neuronal phosphoprotein FLJ20940 IPPD Neuronal phosphoprotein DARPP 32 PPP1R1B PPR1B_HUMAN Protein phosphatase 1 regulatory (inhibitor) subunit 1B Protein phosphatase 1 regulatory inhibitor subunit 1B Protein phosphatase 1 regulatory subunit 1B |
Images
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Fig1:
Western blot analysis of DARPP32 on different lysates with Rabbit anti-DARPP32 antibody (ET1608-23) at 1/500 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 32 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-23) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining of DARPP32 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of DARPP32 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-23, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Flow cytometric analysis of DARPP32 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.