DARPP32 Recombinant Rabbit Monoclonal Antibody [SU0329]
cat.: ET1608-23
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, IHC-Fr, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SU0329 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DARPP32 aa 1-94 / 204. |
| Positive control: | Mouse striatum tissue, mouse forebrain tissue, human brain tissue, human colon tissue, mouse brian tissue, mouse epididymis tissue, rat brian tissue, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Rat hippocampus tissue lysate, SH-SY5Y, MCF-7, Hela. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Tissue |
1:1,000-1:5,000 1:500-1:1,000 1:500 1:500 |
| Uniprot #: | SwissProt: Q9UD71 Human | Q60829 Mouse | Q6J4I0 Rat |
| Alternative names: | DARPP 32 DARPP-32 Dopamine and cAMP regulated neuronal phosphoprotein 32 Dopamine and cAMP regulated neuronal phosphoprotein Dopamine and cAMP regulated phosphoprotein Dopamine and cAMP regulated phosphoprotein DARPP 32 Dopamine and cAMP regulated phosphoprotein DARPP32 Dopamine- and cAMP-regulated neuronal phosphoprotein FLJ20940 IPPD Neuronal phosphoprotein DARPP 32 PPP1R1B PPR1B_HUMAN Protein phosphatase 1 regulatory (inhibitor) subunit 1B Protein phosphatase 1 regulatory inhibitor subunit 1B Protein phosphatase 1 regulatory subunit 1B |
Images
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Fig1:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Striatum Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1608-23, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig2:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Cerebral cortex Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1608-23, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig3:
Application: Immunofluorescence (IHC-Fr) Species: Mouse Tissue: Cerebellum Sample: Frozen section Antigen retrieval: Not required Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ET1608-23, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-DARPP32 antibody (ET1608-23) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-23) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-DARPP32 antibody (ET1608-23) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-23) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Striatum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1608-23, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Rabbit anti-DARPP32 antibody (ET1608-23) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-23) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Striatum Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ET1608-23, 1/1,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig9:
Western blot analysis of DARPP32 on different lysates with Rabbit anti-DARPP32 antibody (ET1608-23) at 1/2,000 dilution. Lane 1: Human brain tissue lysate (40 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Lane 4: Rat hippocampus tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 32 kDa Exposure time: 12 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-23) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.