YAP1 Recombinant Rabbit Monoclonal Antibody [SU33-06]
cat.: ET1608-30
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Clonality: Monoclonal
Clone number: SU33-06
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human YAP1 aa 421-470 / 504.
Positive control: PC-12 cell lysate, rat liver tissue lysate, HeLa, U-2 OS, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, HCT 116 cell lysate, Mouse liver tissue lysate, Mouse kidney tissue lysate, Mouse testis tissue lysate, Mouse skin tissue lysate.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IF-Tissue
  IHC-P
  FC
  IP
1:1,000-1:5,000
1:100-1:500
1:200
1:200
1:1,000
Use at an assay dependent concentration.
Uniprot #: SwissProt: P46937 Human | P46938 Mouse | Q2EJA0 Rat
Alternative names: 65 kDa Yes associated protein 65 kDa Yes-associated protein COB1 YAp 1 YAP 65 YAP YAP1 YAP1_HUMAN YAP2 YAP65 yes -associated protein delta Yes associated protein 1 65kDa Yes associated protein 1 Yes associated protein 2 yes associated protein beta YKI Yorkie homolog
Images
ET1608-30_1.jpg Fig1: Western blot analysis of YAP1 on different lysates with Rabbit anti-YAP1 antibody (ET1608-30) at 1/1,000 dilution.

Lane 1: Mouse liver tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Mouse skin tissue lysate

Lysates/proteins at 40 µg/Lane.

Predicted band size: 54 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-30) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1608-30_2.jpg Fig2: Western blot analysis of YAP1 on different lysates with Rabbit anti-YAP1 antibody (ET1608-30) at 1/1,000 dilution.

Lane 1: PC-12 cell lysate (10 µg/Lane)
Lane 2: Rat liver tissue lysate (20 µg/Lane)

Predicted band size: 54 kDa
Observed band size: 75 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-30) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1608-30_3.jpg Fig3: Western blot analysis of YAP1 on different lysates with Rabbit anti-YAP1 antibody (ET1608-30) at 1/5,000 dilution.

Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1608-30, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 54kDa
Observed band size: 70kDa
ET1608-30_4.jpg Fig4: Western blot analysis of YAP1 on different lysates with Rabbit anti-YAP1 antibody (ET1608-30) at 1/1,000 dilution.

Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si YAP1 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 54 kDa
Observed band size: 75 kDa

Exposure time: 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-30) at 1/1,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ET1608-30_5.jpg Fig5: Application: IF-Tissue

Species: Human

Site: colon carcinoma

Sample: Paraffin-embedded section

Antibody concentration: 1/200
ET1608-30_6.jpg Fig6: Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: HeLa (Human cervix adenocarcinoma epithelial cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: ET1608-30, 1/100, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
ET1608-30_7.jpg Fig7: Application: Immunocytochemistry (IF-cell)

Species: Human
Sample: U-2 OS (Human osteosarcoma cell)

Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
Antibody dilution buffer: 1% BSA in PBST.

Primary antibody: ET1608-30, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature.
Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
ET1608-30_8.jpg Fig8: Flow cytometric analysis of HeLa cells labeling YAP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (ET1608-30, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ET1608-30_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-YAP1 antibody (ET1608-30) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-30) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-30_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-YAP1 antibody (ET1608-30) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-30) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ET1608-30_11.jpg Fig11: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-YAP1 antibody (ET1608-30) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-30) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.