VEGF Receptor 2 Recombinant Rabbit Monoclonal Antibody [SU03-42]
cat.: ET1608-33
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SU03-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 152 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VEGF Receptor 2 aa 30-630 / 1,356. |
| Positive control: | A431, HUVEC, PMVEC, human placenta tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Cell junction, Endoplasmic reticulum, Cell membrane, Early endosome, Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P IP |
1:500-1:1,000 1:50-1:200 1:50-1:100 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P35968 Human | O08775 Rat |
| Alternative names: | CD309 CD309 antigen EC 2.7.10.1 Fetal liver kinase 1 FLK-1 FLK1 FLK1, mouse, homolog of Kdr Kinase insert domain receptor (a type III receptor tyrosine kinase) Kinase insert domain receptor KRD1 Ly73 Protein tyrosine kinase receptor FLK1 Protein-tyrosine kinase receptor flk-1 soluble VEGFR2 Tyrosine kinase growth factor receptor Vascular endothelial growth factor receptor 2 VEGFR 2 VEGFR VEGFR-2 VEGFR2 VGFR2_HUMAN |
Images
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Fig1:
Western blot analysis of VEGF Receptor 2 on different lysates with Rabbit anti-VEGF Receptor 2 antibody (ET1608-33) at 1/1,000 dilution. Lane 1: Human lung tissue lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 150 kDa Observed band size: 150/250 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-33) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining VEGF Receptor 2 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining VEGF Receptor 2 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining VEGF Receptor 2 in PMVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-VEGF Receptor 2 antibody (ET1608-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-VEGF Receptor 2 antibody (ET1608-33) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-33) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.